As you see in attached photo,hard disk's C in primary connectivity server need to defragmentatio(optimize), Is there no problem performing this while the process is running?
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I recommend performing computer maintenance only after a Configuration Wizard > Maintenance Stop.
One of the major reasons why configuring redundant 800xA server roles is to allow a restart or temporary outage of a server.
Trust System 800xA redundancy - and use it when you need!
Defragmenting a disk can be extremely IO intensive, the added latency and potential file locks/access restrictions may cause stress, timeout or worse in System 800xA services.